Pneumococcal hydrogen peroxide regulates host cell kinase activity

INTRODUCTION: Protein kinases are indispensable reversible molecular switches that adapt and control protein functions during cellular processes requiring rapid responses to internal and external events. Bacterial infections can affect kinase-mediated phosphorylation events, with consequences for both innate and adaptive immunity, through regulation of antigen presentation, pathogen recognition, cell invasiveness and phagocytosis. Streptococcus pneumoniae (Spn), a human respiratory tract pathogen and a major cause of community-acquired pneumoniae, affects phosphorylation-based signalling of several kinases, but the pneumococcal mediator(s) involved in this process remain elusive. In this study, we investigated the influence of pneumococcal H(2)O(2) on the protein kinase activity of the human lung epithelial H441 cell line, a generally accepted model of alveolar epithelial cells. METHODS: We performed kinome analysis using PamGene microarray chips and protein analysis in Western blotting in H441 lung cells infected with Spn wild type (SpnWT) or with Spn

• Bazant, J.
• Weiss, A.
• Baldauf, J.
• Schermuly, R. T.
• Hain, T.
• Lucas, R.
• Mraheil, M. A.