Generation of 3D datasets from serial sections of tissues imaged by light microscopy (LM) allows identification of rare structures by morphology or fluorescent labeling. Here, we demonstrate a workflow for correlative light- and electron microscopy (EM) from three-dimensional (3D) LM to 3D EM, using the same sectioned material for both methods consecutively. The new approach is easy to reproduce in routine EM laboratories and applicable to a wide range of organs and research questions.
- Hegermann, J.
- Wrede, C.
- Fassbender, S.
- Schliep, R.
- Ochs, M.
- Knudsen, L.
- Muhlfeld, C.
Keywords
- correlative light and electron microscopy
- fluorescence microscop
- focused ion beam scanning electron microscopy
- tissue clearing
