Alveolar macrophages initiate the spatially targeted recruitment of neutrophils after nanoparticle inhalation

Lung resident immune cells are essential for initiating defenses against inhaled air pollutants, including nanoparticles (NPs), which contribute to pulmonary disease progression. Here, lung intravital microscopy was used to examine the pulmonary innate immune responses in mice, during acute aerosol exposure to carbon NPs, a common environmental pollutant, or fluorescent quantum dot NPs. We found that inhaled NPs triggered rapid, neutrophil recruitment, localized to alveolar NP deposition hotspots, orchestrated by alveolar macrophages (AMs) through both their motility and phagocytic activity. AM motility inhibition in the alveoli via intercellular adhesion molecule-1/LFA-1 blockade reduced neutrophil recruitment, as did impaired AM phagocytosis through C5a receptor 1/Fc-

• Liu, Q.
• Yang, L.
• Li, C.
• Han, L.
• Wilmot, J.
• Yang, G.
• Leinardi, R.
• Zhou, Q.
• Schröppel, A.
• Kutschke, D.
• Secklehner, J.
• Yldirim, AÖ
• Huaux, F.
• Zeuschner, D.
• Carlin, L. M.
• Sperandio, M.
• Schmid, O.
• Stoeger, T.
• Rehberg, M.