Science and Research

High-parametric protein maps reveal the spatial organization in early-developing human lung

The respiratory system, including the lungs, is essential for terrestrial life. While recent research has advanced our understanding of lung development, much still relies on animal models and transcriptome analyses. In this study conducted within the Human Developmental Cell Atlas (HDCA) initiative, we describe the protein-level spatiotemporal organization of the lung during the first trimester of human gestation. Using high-parametric tissue imaging with a 30-plex antibody panel, we analyzed human lung samples from 6 to 13 post-conception weeks, generating data from over 2 million cells across five developmental timepoints. We present a resource detailing spatially resolved cell type composition of the developing human lung, including proliferative states, immune cell patterns, spatial arrangement traits, and their temporal evolution. This represents an extensive single-cell resolved protein-level examination of the developing human lung and provides a valuable resource for further research into the developmental roots of human respiratory health and disease.

  • Sariyar, S.
  • Sountoulidis, A.
  • Hansen, J. N.
  • Marco Salas, S.
  • Mardamshina, M.
  • Martinez Casals, A.
  • Ballllosera Navarro, F.
  • Andrusivova, Z.
  • Li, X.
  • Czarnewski, P.
  • Lundeberg, J.
  • Linnarsson, S.
  • Nilsson, M.
  • Sundström, E.
  • Samakovlis, C.
  • Lundberg, E.
  • Ayoglu, B.

Keywords

  • Humans
  • *Lung/metabolism
  • Female
  • Pregnancy
  • Single-Cell Analysis/methods
  • Pregnancy Trimester, First
Publication details
DOI: 10.1038/s41467-024-53752-x
Journal: Nat Commun
Pages: 9381 
Number: 1
Work Type: Original
Location: UGMLC
Disease Area: General Lung and Other
Partner / Member: JLU
Access-Number: 39477961

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