Science and Research

MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration

Mycofactocin is a redox cofactor essential for the alcohol metabolism of mycobacteria. While the biosynthesis of mycofactocin is well established, the gene mftG, which encodes an oxidoreductase of the glucose-methanol-choline superfamily, remained functionally uncharacterized. Here, we show that MftG enzymes are almost exclusively found in genomes containing mycofactocin biosynthetic genes and are present in 75% of organisms harboring these genes. Gene deletion experiments in Mycolicibacterium smegmatis demonstrated a growth defect of the

  • Graça, A. P.
  • Nikitushkin, V.
  • Ellerhorst, M.
  • Vilhena, C.
  • Klassert, T. E.
  • Starick, A.
  • Siemers, M.
  • Al-Jammal, W. K.
  • Vilotijevic, I.
  • Slevogt, H.
  • Papenfort, K.
  • Lackner, G.

Keywords

  • *Oxidation-Reduction
  • *Ethanol/metabolism
  • *Mycobacterium smegmatis/metabolism/genetics
  • *Bacterial Proteins/metabolism/genetics
  • Gene Deletion
  • Oxidoreductases/metabolism/genetics
  • Peptides
  • Mycofactocin
  • Mycolicibacterium smegmatis
  • biochemistry
  • chemical biology
  • infectious disease
  • microbiology
  • mycobacteria
  • oxidoreductase
  • redox-cofactor
  • respiration
Publication details
DOI: 10.7554/eLife.97559
Journal: Elife
Work Type: Original
Location: BREATH
Disease Area: PALI
Partner / Member: MHH
Access-Number: 39878311

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