Science and Research

Cell-Surface Programmed Death Ligand-1 Expression Identifies a Sub-Population of Distal Epithelial Cells Enriched in Idiopathic Pulmonary Fibrosis

Idiopathic lung fibrosis (IPF) is a fatal lung disease characterized by chronic epithelial injury and exhausted repair capacity of the alveolar compartment, associated with the expansion of cells with intermediate alveolar epithelial cell (AT2) characteristics. Using Sftpc(CreERT2/+): tdTomato(flox/flox) mice, we previously identified a lung population of quiescent injury-activated alveolar epithelial progenitors (IAAPs), marked by low expression of the AT2 lineage trace marker tdTomato (Tom(low)) and characterized by high levels of Pd-l1 (Cd274) expression. This led us to hypothesize that a population with similar properties exists in the human lung. To that end, we used flow cytometry to characterize the CD274 cell-surface expression in lung epithelial cells isolated from donor and end-stage IPF lungs. The identity and functional behavior of these cells were further characterized by qPCR analysis, in vitro organoid formation, and ex vivo precision-cut lung slices (PCLSs). Our analysis led to the identification of a population of CD274(pos) cells expressing intermediate levels of SFTPC, which was expanded in IPF lungs. While donor CD274(pos) cells initiated clone formation, they did not expand significantly in 3D organoids in AT2-supportive conditions. However, an increased number of CD274(pos) cells was found in cultured PCLS. In conclusion, we demonstrate that, similar to IAAPs in the mouse lung, a population of CD274-expressing cells exists in the normal human lung, and this population is expanded in the IPF lung and in an ex vivo PCLS assay, suggestive of progenitor cell behavior. CD274 function in these cells as a checkpoint inhibitor may be crucial for their progenitor function, suggesting that CD274 inhibition, unless specifically targeted, might further injure the already precarious lung epithelial compartment in IPF.

  • Ahmadvand, N.
  • Carraro, G.
  • Jones, M. R.
  • Shalashova, I.
  • Noori, A.
  • Wilhelm, J.
  • Baal, N.
  • Khosravi, F.
  • Chen, C.
  • Zhang, J. S.
  • Ruppert, C.
  • Guenther, A.
  • Wasnick, R. M.
  • Bellusci, S.

Keywords

  • Cd274
  • Iaap
  • Ipf
  • Pd-l1
  • immune privilege
  • lung progenitor
Publication details
DOI: 10.3390/cells11101593
Journal: Cells
Number: 10
Work Type: Original
Location: UGMLC
Disease Area: DPLD
Partner / Member: JLU
Access-Number: 35626630

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