Regulation of T cell tissue residency and activation in human PCLS

BACKGROUND: Resident immune cells are central in shaping the lung's tissue-specific immunity. Precision-cut lung slices (PCLS) preserve the native tissue microenvironment and are therefore an excellent ex vivo model to analyze residency and functionality of resident memory T cells. METHODS: To study the modulation of tissue residency markers and T cell activation in the native lung niche, we treated PCLS with broad and T cell-specific stimuli and analyzed responses using flow cytometry and mediator secretion analysis. Using TGF

• Bargmann, T.
• Sommer, C.
• Stowasser, L.
• Jacob, S.
• Jürgen, L.
• Konzok, S.
• Werlein, C.
• Zardo, P.
• Neubert, L.
• Jonigk, D.
• Fieguth, H. G.
• Dahlmann, F.
• Sewald, K.
• Dehmel, S.
• Braun, A.