Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblasts

BACKGROUND: Fibroblast to myofibroblast conversion is a major driver of tissue remodeling in organ fibrosis. Distinct lineages of fibroblasts support homeostatic tissue niche functions, yet, their specific activation states and phenotypic trajectories during injury and repair have remained unclear. METHODS: We combined spatial transcriptomics, multiplexed immunostainings, longitudinal single-cell RNA-seq and genetic lineage tracing to study fibroblast fates during mouse lung regeneration. Our findings were validated in IPF patient tissues in situ as well as in cell differentiation and invasion assays using patient lung fibroblasts. Cell differentiation and invasion assays established a function of SFRP1 in regulating human lung fibroblast invasion in response to TGF

• Mayr, C. H.
• Sengupta, A.
• Asgharpour, S.
• Ansari, M.
• Pestoni, J. C.
• Ogar, P.
• Angelidis, I.
• Liontos, A.
• Rodriguez-Castillo, J. A.
• Lang, N. J.
• Strunz, M.
• Porras-Gonzalez, D.
• Gerckens, M.
• De Sadeleer, L. J.
• Oehrle, B.
• Viteri-Alvarez, V.
• Fernandez, I. E.
• Tallquist, M.
• Irmler, M.
• Beckers, J.
• Eickelberg, O.
• Stoleriu, G. M.
• Behr, J.
• Kneidinger, N.
• Wuyts, W. A.
• Wasnick, R. M.
• Yildirim, AÖ
• Ahlbrecht, K.
• Morty, R. E.
• Samakovlis, C.
• Theis, F. J.
• Burgstaller, G.
• Schiller, H. B.