Science and Research

Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays

In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. Moreover, such mutations can prevent the binding of mutant probes designed for the identification of specific resistance mutations. Although these mutations are likely rare globally, they occur in approximately 7% of multidrug-resistant tuberculosis strains in some settings.

  • Ajileye, A.
  • Alvarez, N.
  • Merker, M.
  • Walker, T. M.
  • Akter, S.
  • Brown, K.
  • Moradigaravand, D.
  • Schon, T.
  • Andres, S.
  • Schleusener, V.
  • Omar, S. V.
  • Coll, F.
  • Huang, H.
  • Diel, R.
  • Ismail, N.
  • Parkhill, J.
  • de Jong, B. C.
  • Peto, T. E.
  • Crook, D. W.
  • Niemann, S.
  • Robledo, J.
  • Smith, E. G.
  • Peacock, S. J.
  • Koser, C. U.


  • Antitubercular Agents/*pharmacology
  • Biological Assay
  • DNA Gyrase/*genetics/metabolism
  • Drug Resistance, Multiple, Bacterial/*genetics
  • False Positive Reactions
  • Fluoroquinolones/*pharmacology
  • Gene Expression
  • Humans
  • *Mutation
  • Mycobacterium tuberculosis/classification/drug effects/*genetics/isolation &
  • purification
  • Oligonucleotide Probes/chemistry/metabolism
  • Phylogeny
  • Tuberculosis, Multidrug-Resistant/drug therapy/microbiology
  • *Hain GenoType MTBDRsl
  • *Mycobacterium tuberculosis
  • *fluoroquinolones
Publication details
DOI: 10.1128/AAC.02169-16
Journal: Antimicrobial agents and chemotherapy
Number: 4
Work Type: Original
Location: Assoziierter Partner, ARCN
Disease Area: General Lung and Other
Partner / Member: UKSH (Kiel)
Access-Number: 28137812
See publication on PubMed

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