Science and Research

Evaluating acellular versus cellular perfusate composition during prolonged ex vivo lung perfusion after initial cold ischaemia for 24 hours

Normothermic ex vivo lung perfusion (EVLP) has developed as a powerful technique to evaluate particularly marginal donor lungs prior to transplantation. In this study, acellular and cellular perfusate compositions were compared in an identical experimental setting as no consensus has been reached on a preferred technique yet. Porcine lungs underwent EVLP for 12 h on the basis of an acellular or a cellular perfusate composition after 24 h of cold ischaemia as defined organ stress. During perfusion, haemodynamic and respiratory parameters were monitored. After EVLP, the lung condition was assessed by light and transmission electron microscopy. Aerodynamic parameters did not show significant differences between groups and remained within the in vivo range during EVLP. Mean oxygenation indices were 491 +/- 39 in the acellular group and 513 +/- 53 in the cellular group. Groups only differed significantly in terms of higher pulmonary artery pressure and vascular resistance in the cellular group. Lung histology and ultrastructure were largely well preserved after prolonged EVLP and showed only minor structural alterations which were similarly present in both groups. Prolonged acellular and cellular EVLP for 12 h are both feasible with lungs prechallenged by ischaemic organ stress. Physiological and ultrastructural analysis showed no superiority of either acellular or cellular perfusate composition.

  • Becker, S.; Steinmeyer, J.; Avsar, M.; Hoffler, K.; Salman, J.; Haverich, A.; Warnecke, G.; Ochs, M.; Schnapper, A.


  • Animals
  • Cold Ischemia/*methods
  • Disease Models, Animal
  • Extracorporeal Circulation/*methods
  • Female
  • Flow Cytometry/methods
  • Graft Rejection
  • Graft Survival
  • Immunohistochemistry
  • Lung/*pathology
  • Lung Transplantation/adverse effects/*methods
  • Organ Preservation/*methods
  • Organ Preservation Solutions/pharmacology
  • Perfusion/methods
  • Random Allocation
  • Risk Assessment
  • Sensitivity and Specificity
  • Sus scrofa
  • Swine
  • Time Factors
  • Tissue Donors
  • acellular perfusion
  • cellular perfusion
  • ex vivo lung perfusion
  • large animal model
  • lung physiology
  • lung ultrastructure
Publication details
DOI: 10.1111/tri.12649
Journal: Transplant international : official journal of the European Society for Organ Transplantation
Pages: 88-97 
Number: 1
Work Type: Original
Location: BREATH
Disease Area: ROR
Partner / Member: MHH
Access-Number: 26264867
See publication on PubMed

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