Streptococcus pyogenes EVs induce the alternative inflammasome via caspase-4/-5 in human monocytes

The sensing of Gram-negative Extracellular Vesicles (EVs) by the innate immune system has been extensively studied in the past decade. In contrast, recognition of Gram-positive EVs by innate immune cells remains poorly understood. Comparative genome-wide transcriptional analysis in human monocytes uncovered that S. pyogenes EVs induce proinflammatory signatures that are markedly distinct from those of their parental cells. Among the 209 genes exclusively upregulated by EVs, caspase-5 prompted us to study inflammasome signaling pathways in depth. We show that lipoteichoic acid (LTA), a structural component of Gram-positive bacterial membranes present on EVs from S. pyogenes and other Gram-positive species, is sensed by TLR2 which triggers the alternative inflammasome composed of NLRP3 and the inflammatory caspases-4/-5 to mount an IL-1

• Krause, K.
• Franch Arroyo, S.
• Ugolini, M.
• Kueck, T.
• Sullivan, T. J.
• Gálvez, E. J. C.
• Muenzner, M.
• Goosmann, C.
• Brinkmann, V.
• Frese, C. K.
• Alagesan, K.
• Vierbuchen, T.
• Heine, H.
• Resch, U.
• Sander, L. E.
• Charpentier, E.