Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing

The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway.

• Merkert, S.
• Haase, A.
• Dahlmann, J.
• Göhring, G.
• Waqas, F. H.
• Pessler, F.
• Martin, U.
• Olmer, R.