New Delhi Metallo-beta-Lactamase (blaNDM) acquisition by Gram-negative bacteria is a primary concern due to its broad host-range distribution. We investigated two potential in vivo horizontal gene transfer (HGT) of blaNDM between Enterobacteriaceae and Pseudomonas aeruginosa which was initially indicated by a PCR diagnostic test. Whole genome sequencing (WGS) showed independent parallel acquisition of two different blaNDM variants (NDM-1 and NDM-5) respectively in P. aeruginosa and Enterobacteriaceae. Our data demonstrated that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest and thus to provide a timely response to potential outbreaks.
- Kocer, K.
- Boutin, S.
- Probst, K.
- Heeg, K.
- Nurjadi, D.
Keywords
- Hgt
- Mdro
- Wgs
- bla(NDM)
- outbreak management
- plasmid
