New Delhi metallo-β-lactamase (bla(NDM)) acquisition by Gram-negative bacteria is a primary concern due to its broad-host-range distribution. This study investigated two potential in-vivo horizontal gene transfers (HGTs) of bla(NDM) between Enterobacterales and Pseudomonas aeruginosa, initially indicated by polymerase chain reaction. Whole-genome sequencing showed independent parallel acquisition of two different bla(NDM) variants (NDM-1 and NDM-5) in P. aeruginosa and Enterobacterales, respectively. The data show that short-read sequencing provides the necessary resolution to confirm or dispute HGT by the comparison of genetic elements surrounding the gene of interest, and thus provide a timely response to potential outbreaks.
- Kocer, K.
- Boutin, S.
- Probst, K.
- Heeg, K.
- Nurjadi, D.
Keywords
- Aged
- Anti-Bacterial Agents/pharmacology
- Coinfection/*microbiology
- Drug Resistance, Multiple, Bacterial/*genetics
- Enterobacteriaceae/drug effects/*genetics
- *Gene Transfer, Horizontal
- Gram-Negative Bacterial Infections/microbiology
- Hospitalization
- Humans
- Male
- Middle Aged
- Plasmids/genetics
- Pseudomonas Infections/microbiology
- Pseudomonas aeruginosa/drug effects/enzymology/*genetics
- Retrospective Studies
- Whole Genome Sequencing
- beta-Lactamases/genetics
- Horizonal gene transfer
- Multi-drug-resistant organism
- Outbreak management
- Plasmid
- Whole-genome sequencing
- bla(NDM)
