Several studies report an important role of CD8(+) cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4(+) T-cell subtypes. Additional effects of CD8(+) T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N = 76) German children. CD3(+) CD8(+) CD25(+) T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8(+) T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farm children, asthmatics displayed increased CD3(+) CD8(low)(CD25(+)) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3(+) CD8(low)(CD25(+)) cells and a lower IFN-gamma secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3(+) CD8(low) cells than non-asthmatics. Our data indicates a specific role of CD8(low) T-cells in asthmatic farm children. (C) 2017 Elsevier Inc. All rights reserved.
- Twardziok, M.
- Schroder, P. C.
- Krusche, J.
- Casaca, V. I.
- Illi, S.
- Bock, A.
- Loss, G. J.
- Kabesch, M.
- Toncheva, A. A.
- Roduit, C.
- Depner, M.
- Genuneit, J.
- Renz, H.
- Roponen, M.
- Weber, J.
- Braun-Fahrlander, C.
- Riedler, J.
- Lauener, R.
- Vuitton, D. A.
- Dalphin, J. C.
- Pekkanen, J.
- von Mutius, E.
- Schaub, B.
- Pasture Study Group
- Hyvarinen, A.
- Karvonen, A. M.
- Kirjavainen, P. V.
- Remes, S.
- Kaulek, V.
- Dalphin, M. L.
- Ege, M.
- Pfefferle, P. I.
- Doekes, G.
Keywords
- cd8(+)
- cd8(low)
- childhood
- farm-exposure
- brown-norway rats
- allergic disease
- childhood asthma
- exposure
- expression
- phenotype
- wheeze
- risk
- age
- differentiation
