α-Glucosyl diacylglycerols (αGlc-DAGs) play an important role in providing protective immunity against Streptococcus pneumoniae infection through the engagement of the Macrophage inducible C-type lectin (Mincle). Herein, we efficiently synthesised αGlc-DAGs containing C12, C14, C16 and C18 acyl chains in 7 steps and 44-47% overall yields, and demonstrated that Mincle signaling was dependent on lipid length using mMincle and hMincle NFAT-GFP reporter cells. The greatest production of GFP in both cell types was elicited by C14 αGlc-DAG. Accordingly, C14 αGlc-DAG has potential to act as an adjuvant to augment the immune response against S. pneumoniae antigens.
- Khan, A.
- Hollwedel, F.
- Maus, U. A.
- Stocker, B. L.
- Timmer, M. S. M.
Keywords
- Glycolipids/chemical synthesis/chemistry/*immunology
- Molecular Structure
- Pneumococcal Infections/*immunology/therapy
- Pneumococcal Vaccines/chemical synthesis/chemistry/*immunology
- Streptococcus pneumoniae/*immunology
- Vaccines, Conjugate/chemistry/*immunology
- interests to report in relation to the submission of our manuscript:‘Synthesis of
- α-Glucosyl Diacylglycerides as potential adjuvants for Streptococcus pneumoniae
- vaccines.’
