Science and Research

The Peculiar Structure of Acetobacter pasteurianus CIP103108 LPS Core Oligosaccharide

Acetobacter pasteurianus, a member of the Alphaproteobacteria, is an acetic acid-producing bacterium present on sugar-rich substrates such as such as fruits, flowers and vegetables and traditionally used in the production of fermented food. The preferred living habitat associated with acid conditions makes the structure of the bacterial cell wall interesting to study, due to expected uncommon features. We have used a combination of chemical, analytical and NMR spectroscopy approaches to define the complete structure of the core oligosaccharide from A. pasteurianus CIP103108 LPS. Interestingly, the core oligosaccharide displays a high concentration of negatively charged groups, structural features that might contribute to reinforcing the bacterial membrane.

  • Marchetti, R.
  • Nieto Fabregat, F.
  • Pallach, M.
  • Gully, D.
  • Giraud, E.
  • Molinaro, A.
  • Duda, K. A.
  • Silipo, A.

Keywords

  • Acetobacter/*chemistry/metabolism
  • Carbohydrate Conformation
  • Lipopolysaccharides/*chemistry/metabolism
  • Nuclear Magnetic Resonance, Biomolecular
  • *Acetobacter pasteurianus
  • *NMR spectroscopy
  • *acetic acid bacteria
  • *core oligosaccharide
  • *lipopolysaccharide
Publication details
DOI: 10.1002/cbic.202000597
Journal: Chembiochem
Pages: 147-150 
Number: 1
Work Type: Original
Location: ARCN
Disease Area: AA
Partner / Member: FZB
Access-Number: 32965769


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